Genome Editing Solutions

Unlock  your genetic research with a CRISPR expert

Genome Editing Solutions

Unlock the potential of your genetic research with a CRISPR expert

About me

I am a passionate scientist and entrepreneur who aims to harness the potential of CRISPR technology to generate a significant global impact. With over 10 years of experience in both industry and academia, my main goal is to develop innovative solutions and assist others in overcoming their unique challenges in genome editing.

Services 

I assist scientists in designing genome editing experiments, I offer customized solutions that are tailored to specific needs and models (animals, plants, cells), providing comprehensive assistance throughout the entire genome editing process. – ¡Contact me for more information!

Experimental Design

  • Design of customized experiments to achieve effective CRISPR genome editing.

  • Strategies for Knock-out, Knock-in, and HDR.

  • Selection of the most suitable editing tool for your project.

  • Optimal gRNA design for your gene of interest (based on algorithms).

  • Selection of the most appropriate delivery method according to your needs.

Data Analysis

  • Analysis and interpretation of genome editing data.

  • Analysis of genotypes and phenotypes of the mutants.

  • Sequencing data analysis.

  • Evaluation and analysis of project risks.

Vector Construction

  • Design of CRISPR plasmids tailored to your project.

  • Optimization of your vector of interest to enhance its efficiency.

  • Design of customized vectors that align with your objectives.

Transgenic organisms

  • Comprehensive guidance from the plasmid construction stage.

  • Advice on the delivery method and event detection.

  • Selection of the appropriate strategy (random vs precise integration).

Tool kit

Cas9, Cas12a (Cpf1), Cas13, SpCas9, SpRYCas9

Gene knock-out (KO) and knock-in (KI)

Homologous Recombination (HDR)

gRNA design and validation

Base Editing (ABE, CBE)

Prime Editing

Gene tagging

How do I work?

  • I apply my knowledge and proven experience in genome editing.

  • I offer personalized guidance, working side by side with you.

  • I provide continuous guidance and listen empathetically throughout the process.

  • I adapt to your needs with customized classes.

Newsletter

Biotechnology Everywhere

Relevant Publications

Brd4 and P300 Confer Transcriptional Competency during Zygotic Genome Activation (2019). S.H. Chan, Y.Tang, L.Miao, H. Codore, C. Vejnar, J.D Beaudoin, D. Musaev, J.P. Fernandez, M. Benitez, A. Bazzini, M.A. Moreno-Mateos, A.J. Giraldez. Dev Cell. PMID: 31211993 .

Optimized CRISPR-Cpf1 system for genome editing in zebrafish. J.P. Fernandez*, C. Vejnar*, A. Giraldez, R. Rouet, M.A. Moreno-Mateos. (2018). Methods PMID: 29964176.

RES complex is involved in intron definition and essential for brain development in vertebrates. J.P. Fernandez*, M.A. Moreno-Mateos*, A. Gohr, S.H. Chan, M. Irimia, A.J. Giraldez (2018). PLoS Genetics PMID: 29969449.

CRISPR-Cpf1 mediates efficient homologous-directed repair and temperature-controlled genome editing. Moreno-Mateos M, J.P. Fernandez, R. Rouet, M. Lane, C. Vejnar, E. Mis, M. Khokha, J. Doudna, A. Giraldez (2017). Nat.Com. PMID: 29222508

CRISPRscan: designing highly efficient sgRNAs for CRISPR/Cas9 targeting in vivo. M.A. Moreno-Mateos*, C.E. Vejnar*, J.D. Beaudoin, J.P. Fernandez, E.K. Mis, M.K. Khokha, A.J. Giraldez (2015). Nature Methods PMID: 26322839.

RES complex is involved in intron definition and essential for brain development in vertebrates. J.P. Fernandez*, M.A. Moreno-Mateos*, A. Gohr, S.H. Chan, M. Irimia, A.J. Giraldez (2018). PLoS Genetics PMID: 29969449.

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